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Order NO. D199971
小鼠抗eCFP单克隆抗体
英文名 Anti-eCFP mouse monoclonal antibody
相关类别 标签抗体 储存 冷冻(-20℃)
宿主 Mouse 反应种属 eCFP
标记物 Unconjugate 克隆类型 mouse monoclonal
抗原 eCFP    
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
D199971-0100 100 ul 预计5日内发货
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技术规格

Background: Cyan Fluorescent Protein (CFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. CFP can be excited by the 405 nm laser line and is optimally detected at 485 nm.
Applications: WB
Name of antibody: eCFP
Immunogen: Purified mCFP protein expressed in HEK293 cell
Full name: Cyan Fluorescent Protein
WB Predicted band size: 27 kDa
WB Positive control: HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY eCFP ne) cDNA for 48 hrs and lysed
WB Recommended dilution: 1000-5000
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Sample Application

  • Gel: 12%SDS-PAGE, Lysate: 5μg, Lane 1-2: HEK293T cells lysates were transfected with the pCMV6-ENTRY control and pCMV6-ENTRY eCFP cDNA for 48 hrs, Primary antibody: D199971(eCFP Antibody) at dilution 1/2000, Secondary antibody: Goat anti mouse IgG at 1/8000 dilution, Exposure time: 2 minutes
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