网 站
反 馈
网 站
指 南
Order NO. C100314
3-马来酰亚胺基苯甲酸琥珀酰亚胺酯(水溶性)
英文名 Sulfo-MBS
别名 3-Maleimidobenzoyl-N-hydroxysulfosuccinimide ester
相关类别 蛋白异源双功能交链试剂 储存 冷冻(-20℃) 避光+干燥
分子量 416.2 3-马来酰亚胺基苯甲酸琥珀酰亚胺酯(水溶性)
   
编 号 包装 库存 目录价(¥) 您的价格(¥) 数 量
C100314-0050 50 MG 现货
加入购物车
产品描述

概述

MBS and its water-soluble analog Sulfo-MBS are heterobifunctional cross-linkers that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7-9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. In aqueous solutions, hydrolytic degradation of the NHS ester is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group but will slowly hydrolyze and also lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugation experiments involving these cross-linkers are usually performed at pH 7.2-7.5, with the NHS-ester (amine-targeted) reaction being accomplished before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.

应用

Sulfo-MBS is soluble in water and many other aqueous buffers to approximately 10 mM, although solubility decreases with increasing salt concentration

组份

50 mg/bottle

技术规格

Molecular Weight 416.3
Spacer arm 7.3
Purity >95% on TLC
相关文档
3499拉斯维加斯官网的购物车(0) 结算
XML 地图 | Sitemap 地图